關鍵詞：maize mays leaf materials primary 

摘要：While being one of the world's most important crops,maize ( Zea mays L.) is still difficult to regenerate in tissue culture which severely limits its improvement by genetic engineering.Currently,immature zygotic embryos provide the predominantly used material for regeneration and transformation.However,the procedures involved are often laborious,time-consuming and season-dependent.Here,we further improved an efficient tissue culture and plant regeneration system that uses maize leaf segments of young seedlings as an alternative explant source.Embryogenic calli were evaluated by morphology,proliferation and regeneration capacity.All these indicated that seedling-derived leaf materials have the potential to replace immature embryos for tissue culture and regeneration.

生物技術雜志要求:

{1}借喻詞一般需用“”號；課題名用“”號，不用“《》”。

{2}來稿一般在1個月內提出處理意見，請不要一稿兩投，若1個月后仍未收到稿件處理通知，作者有權自行處理來稿。

{3}參考文獻著錄采用數字加方括號編序集中列于文后，其著錄格式如下：序號、主要作者、文獻及載體、出版項（出版者、出版年月）。

{4}中、英文關鍵詞3～8個，中文關鍵詞間空1個字距，不用任何標點符號，英文關鍵詞用分號(；)隔開。

{5}作者姓名的漢語拼音應姓前名后，姓氏全部字母為大寫，名字的首字母大寫，雙名中間不加連字符不間隔；漢語拼音的作者姓名寫在英文表示的工作單位之前；多位作者的署名之間以逗號隔開；不同單位的作者姓名右上角加注序號。

注：因版權方要求，不能公開全文，如需全文，請咨詢雜志社